Construct: shRNA TRCN0000343808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015474.2-1089s21c1
DNA Barcode:
GCCATCATCTTGGAATCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMHD1 (25939)
Gene Description:
SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
Transcript:
RefSeq NM_015474.2 (NON-CURRENT)
Match location:
Position 1089 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCATCTTGGAATCCAAA
Hairpin Sequence:
5'-CCGG-GCCATCATCTTGGAATCCAAA-CTCGAG-TTTGGATTCCAAGATGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCATCTTGGAATCCAAACTCGAGTTTGGATTCCAAGATGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCATCTTGGAATCCAAACTCGAGTTTGGATTCCAAGATGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000142564