Construct: shRNA TRCN0000343909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022060.2-525s21c1
DNA Barcode:
CACTTCTTACTCAATCAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABHD4 (63874)
Gene Description:
abhydrolase domain containing 4
Transcript:
RefSeq NM_022060.2 (CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTCTTACTCAATCAAGTA
Hairpin Sequence:
5'-CCGG-CACTTCTTACTCAATCAAGTA-CTCGAG-TACTTGATTGAGTAAGAAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTCTTACTCAATCAAGTACTCGAGTACTTGATTGAGTAAGAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTCTTACTCAATCAAGTACTCGAGTACTTGATTGAGTAAGAAGTG-3'

Other clones with same target sequence:

  1. TRCN0000049931