Construct: shRNA TRCN0000343926

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006726.2-8795s21c1
DNA Barcode:
CCACGATGGAAACAGATGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRBA (987)
Gene Description:
LPS responsive beige-like anchor protein
Transcript:
RefSeq NM_006726.2 (NON-CURRENT)
Match location:
Position 8795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000343926)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGATGGAAACAGATGATA
Hairpin Sequence:
5'-CCGG-CCACGATGGAAACAGATGATA-CTCGAG-TATCATCTGTTTCCATCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGATGGAAACAGATGATACTCGAGTATCATCTGTTTCCATCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGATGGAAACAGATGATACTCGAGTATCATCTGTTTCCATCGTGG-3'

Other clones with same target sequence:

  1. TRCN0000148506