Construct: shRNA TRCN0000344114

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001483.1-1818s21c1
DNA Barcode:
CCATGTGAAAGTCCTGTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NIPSNAP2 (2631)
Gene Description:
nipsnap homolog 2
Transcript:
RefSeq NM_001483.1 (NON-CURRENT)
Match location:
Position 1818 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344114)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGAAAGTCCTGTTGAA
Hairpin Sequence:
5'-CCGG-CCATGTGAAAGTCCTGTTGAA-CTCGAG-TTCAACAGGACTTTCACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGAAAGTCCTGTTGAACTCGAGTTCAACAGGACTTTCACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGAAAGTCCTGTTGAACTCGAGTTCAACAGGACTTTCACATGG-3'

Other clones with same target sequence:

  1. TRCN0000135683