Construct: shRNA TRCN0000344135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173638.2-2810s21c1
DNA Barcode:
GCATGTCTCTGAGCTTCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBPF15 (284565)
Gene Description:
NBPF member 15
Transcript:
RefSeq NM_173638.2 (NON-CURRENT)
Match location:
Position 2810 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTCTCTGAGCTTCTATA
Hairpin Sequence:
5'-CCGG-GCATGTCTCTGAGCTTCTATA-CTCGAG-TATAGAAGCTCAGAGACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTCTCTGAGCTTCTATACTCGAGTATAGAAGCTCAGAGACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTCTCTGAGCTTCTATACTCGAGTATAGAAGCTCAGAGACATGC-3'

Other clones with same target sequence:

(none)