Construct: shRNA TRCN0000344227

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177985.2-571s21c1
DNA Barcode:
ATGGATGATGTCACGACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARL5A (26225)
Gene Description:
ADP ribosylation factor like GTPase 5A
Transcript:
RefSeq NM_177985.2 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344227)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGATGATGTCACGACTTAA
Hairpin Sequence:
5'-CCGG-ATGGATGATGTCACGACTTAA-CTCGAG-TTAAGTCGTGACATCATCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGATGATGTCACGACTTAACTCGAGTTAAGTCGTGACATCATCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGATGATGTCACGACTTAACTCGAGTTAAGTCGTGACATCATCCAT-3'

Other clones with same target sequence:

(none)