Construct: shRNA TRCN0000344247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014988.2-1460s21c1
DNA Barcode:
GCCATTCAACAGAGCCAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIMCH1 (22998)
Gene Description:
LIM and calponin homology domains 1
Transcript:
RefSeq NM_014988.2 (NON-CURRENT)
Match location:
Position 1460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTCAACAGAGCCAAATT
Hairpin Sequence:
5'-CCGG-GCCATTCAACAGAGCCAAATT-CTCGAG-AATTTGGCTCTGTTGAATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTCAACAGAGCCAAATTCTCGAGAATTTGGCTCTGTTGAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTCAACAGAGCCAAATTCTCGAGAATTTGGCTCTGTTGAATGGC-3'

Other clones with same target sequence:

  1. TRCN0000153260