Construct: shRNA TRCN0000344276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001122646.1-2354s21c1
DNA Barcode:
ACTGGAGGAAGCGAAGCATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM178B (51252)
Gene Description:
family with sequence similarity 178 member B
Transcript:
RefSeq NM_001122646.1 (NON-CURRENT)
Match location:
Position 2354 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGAGGAAGCGAAGCATCA
Hairpin Sequence:
5'-CCGG-ACTGGAGGAAGCGAAGCATCA-CTCGAG-TGATGCTTCGCTTCCTCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGAGGAAGCGAAGCATCACTCGAGTGATGCTTCGCTTCCTCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGAGGAAGCGAAGCATCACTCGAGTGATGCTTCGCTTCCTCCAGT-3'

Other clones with same target sequence:

(none)