Construct: shRNA TRCN0000344345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_203463.1-1298s21c1
DNA Barcode:
CCCAAGCAAAGTGAACTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERS6 (253782)
Gene Description:
ceramide synthase 6
Transcript:
RefSeq NM_203463.1 (NON-CURRENT)
Match location:
Position 1298 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGCAAAGTGAACTATTT
Hairpin Sequence:
5'-CCGG-CCCAAGCAAAGTGAACTATTT-CTCGAG-AAATAGTTCACTTTGCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGCAAAGTGAACTATTTCTCGAGAAATAGTTCACTTTGCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGCAAAGTGAACTATTTCTCGAGAAATAGTTCACTTTGCTTGGG-3'

Other clones with same target sequence:

(none)