Construct: shRNA TRCN0000344408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001145132.1-523s21c1
DNA Barcode:
GGATACCACCACCAGTTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C5orf52 (100190949)
Gene Description:
chromosome 5 open reading frame 52
Transcript:
RefSeq NM_001145132.1 (NON-CURRENT)
Match location:
Position 523 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATACCACCACCAGTTTAAA
Hairpin Sequence:
5'-CCGG-GGATACCACCACCAGTTTAAA-CTCGAG-TTTAAACTGGTGGTGGTATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATACCACCACCAGTTTAAACTCGAGTTTAAACTGGTGGTGGTATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATACCACCACCAGTTTAAACTCGAGTTTAAACTGGTGGTGGTATCC-3'

Other clones with same target sequence:

(none)