Construct: shRNA TRCN0000344449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001159293.1-1103s21c1
DNA Barcode:
GCGGAGAGAAACCCTACAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF737 (100129842)
Gene Description:
zinc finger protein 737
Transcript:
RefSeq NM_001159293.1 (NON-CURRENT)
Match location:
Position 1103 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAGAGAAACCCTACAAAT
Hairpin Sequence:
5'-CCGG-GCGGAGAGAAACCCTACAAAT-CTCGAG-ATTTGTAGGGTTTCTCTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAGAGAAACCCTACAAATCTCGAGATTTGTAGGGTTTCTCTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAGAGAAACCCTACAAATCTCGAGATTTGTAGGGTTTCTCTCCGC-3'

Other clones with same target sequence:

(none)