Construct: shRNA TRCN0000344593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032663.3-1910s21c1
DNA Barcode:
CTCATCTGATACAGGTAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP30 (84749)
Gene Description:
ubiquitin specific peptidase 30
Transcript:
RefSeq NM_032663.3 (NON-CURRENT)
Match location:
Position 1910 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCTGATACAGGTAATTA
Hairpin Sequence:
5'-CCGG-CTCATCTGATACAGGTAATTA-CTCGAG-TAATTACCTGTATCAGATGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCTGATACAGGTAATTACTCGAGTAATTACCTGTATCAGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCTGATACAGGTAATTACTCGAGTAATTACCTGTATCAGATGAG-3'

Other clones with same target sequence:

(none)