Construct: shRNA TRCN0000344602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005607.3-4258s21c1
DNA Barcode:
CCCAGGAGAGAATGAAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Gene Description:
protein tyrosine kinase 2
Transcript:
RefSeq NM_005607.3 (NON-CURRENT)
Match location:
Position 4258 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGAGAGAATGAAGCAAA
Hairpin Sequence:
5'-CCGG-CCCAGGAGAGAATGAAGCAAA-CTCGAG-TTTGCTTCATTCTCTCCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGAGAGAATGAAGCAAACTCGAGTTTGCTTCATTCTCTCCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGAGAGAATGAAGCAAACTCGAGTTTGCTTCATTCTCTCCTGGG-3'

Other clones with same target sequence:

  1. TRCN0000121207