Construct: shRNA TRCN0000344800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001107.3-413s21c1
DNA Barcode:
ATAAACTCAGTGGTTTGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACYP1 (97)
Gene Description:
acylphosphatase 1
Transcript:
RefSeq NM_001107.3 (NON-CURRENT)
Match location:
Position 413 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAAACTCAGTGGTTTGGTTT
Hairpin Sequence:
5'-CCGG-ATAAACTCAGTGGTTTGGTTT-CTCGAG-AAACCAAACCACTGAGTTTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAAACTCAGTGGTTTGGTTTCTCGAGAAACCAAACCACTGAGTTTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAAACTCAGTGGTTTGGTTTCTCGAGAAACCAAACCACTGAGTTTAT-3'

Other clones with same target sequence:

(none)