Construct: shRNA TRCN0000344808

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006212.2-1169s21c1
DNA Barcode:
GAGAAGTATCTGTATCGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKFB2 (5208)
Gene Description:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
Transcript:
RefSeq NM_006212.2 (CURRENT)
Match location:
Position 1169 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344808)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGTATCTGTATCGATAT
Hairpin Sequence:
5'-CCGG-GAGAAGTATCTGTATCGATAT-CTCGAG-ATATCGATACAGATACTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGTATCTGTATCGATATCTCGAGATATCGATACAGATACTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGTATCTGTATCGATATCTCGAGATATCGATACAGATACTTCTC-3'

Other clones with same target sequence:

  1. TRCN0000196381