Construct: shRNA TRCN0000344809

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033550.3-1065s21c1
DNA Barcode:
GATCTAAGTAAAGGTGTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53RK (112858)
Gene Description:
TP53 regulating kinase
Transcript:
RefSeq NM_033550.3 (NON-CURRENT)
Match location:
Position 1065 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344809)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTAAGTAAAGGTGTTAAG
Hairpin Sequence:
5'-CCGG-GATCTAAGTAAAGGTGTTAAG-CTCGAG-CTTAACACCTTTACTTAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTAAGTAAAGGTGTTAAGCTCGAGCTTAACACCTTTACTTAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTAAGTAAAGGTGTTAAGCTCGAGCTTAACACCTTTACTTAGATC-3'

Other clones with same target sequence:

  1. TRCN0000196427