Construct: shRNA TRCN0000344934

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024665.4-1051s21c1
DNA Barcode:
CAGCATAAAGGCCCTATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBL1XR1 (79718)
Gene Description:
transducin beta like 1 X-linked receptor 1
Transcript:
RefSeq NM_024665.4 (NON-CURRENT)
Match location:
Position 1051 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344934)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCATAAAGGCCCTATATTT
Hairpin Sequence:
5'-CCGG-CAGCATAAAGGCCCTATATTT-CTCGAG-AAATATAGGGCCTTTATGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCATAAAGGCCCTATATTTCTCGAGAAATATAGGGCCTTTATGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCATAAAGGCCCTATATTTCTCGAGAAATATAGGGCCTTTATGCTG-3'

Other clones with same target sequence:

(none)