Construct: shRNA TRCN0000344974

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013890.3-1594s21c1
DNA Barcode:
GAACCAAAGTTCTCACCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxw2 (30050)
Gene Description:
F-box and WD-40 domain protein 2
Transcript:
RefSeq NM_013890.3 (NON-CURRENT)
Match location:
Position 1594 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344974)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCAAAGTTCTCACCTAAA
Hairpin Sequence:
5'-CCGG-GAACCAAAGTTCTCACCTAAA-CTCGAG-TTTAGGTGAGAACTTTGGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCAAAGTTCTCACCTAAACTCGAGTTTAGGTGAGAACTTTGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCAAAGTTCTCACCTAAACTCGAGTTTAGGTGAGAACTTTGGTTC-3'

Other clones with same target sequence:

  1. TRCN0000012813