Construct: shRNA TRCN0000344984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008854.4-1181s21c1
DNA Barcode:
CACGAGTAACTTTGACGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prkaca (18747)
Gene Description:
protein kinase, cAMP dependent, catalytic, alpha
Transcript:
RefSeq NM_008854.4 (NON-CURRENT)
Match location:
Position 1181 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000344984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGAGTAACTTTGACGACTA
Hairpin Sequence:
5'-CCGG-CACGAGTAACTTTGACGACTA-CTCGAG-TAGTCGTCAAAGTTACTCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGAGTAACTTTGACGACTACTCGAGTAGTCGTCAAAGTTACTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGAGTAACTTTGACGACTACTCGAGTAGTCGTCAAAGTTACTCGTG-3'

Other clones with same target sequence:

  1. TRCN0000012462