Construct: shRNA TRCN0000345000

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182994.2-445s21c1
DNA Barcode:
GCCAAGAATCACTTCGTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arl5a (75423)
Gene Description:
ADP-ribosylation factor-like 5A
Transcript:
RefSeq NM_182994.2 (CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345000)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAATCACTTCGTCCTT
Hairpin Sequence:
5'-CCGG-GCCAAGAATCACTTCGTCCTT-CTCGAG-AAGGACGAAGTGATTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAATCACTTCGTCCTTCTCGAGAAGGACGAAGTGATTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAATCACTTCGTCCTTCTCGAGAAGGACGAAGTGATTCTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000011944