Construct: shRNA TRCN0000345008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018757.1-831s21c1
DNA Barcode:
GCTGCAGTCCAGTTCTATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nme6 (54369)
Gene Description:
NME/NM23 nucleoside diphosphate kinase 6
Transcript:
RefSeq NM_018757.1 (CURRENT)
Match location:
Position 831 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAGTCCAGTTCTATCTA
Hairpin Sequence:
5'-CCGG-GCTGCAGTCCAGTTCTATCTA-CTCGAG-TAGATAGAACTGGACTGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAGTCCAGTTCTATCTACTCGAGTAGATAGAACTGGACTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAGTCCAGTTCTATCTACTCGAGTAGATAGAACTGGACTGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000221702