Construct: shRNA TRCN0000345026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198703.1-6257s21c1
DNA Barcode:
CCGTCCAGAATCTAAGTCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wnk1 (232341)
Gene Description:
WNK lysine deficient protein kinase 1
Transcript:
RefSeq NM_198703.1 (NON-CURRENT)
Match location:
Position 6257 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCCAGAATCTAAGTCAAA
Hairpin Sequence:
5'-CCGG-CCGTCCAGAATCTAAGTCAAA-CTCGAG-TTTGACTTAGATTCTGGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCCAGAATCTAAGTCAAACTCGAGTTTGACTTAGATTCTGGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCCAGAATCTAAGTCAAACTCGAGTTTGACTTAGATTCTGGACGG-3'

Other clones with same target sequence:

  1. TRCN0000027001