Construct: shRNA TRCN0000345208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023341.3-1818s21c1
DNA Barcode:
ATGCTCACCTGGATGCTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coq8a (67426)
Gene Description:
coenzyme Q8A
Transcript:
RefSeq NM_023341.3 (CURRENT)
Match location:
Position 1818 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGCTCACCTGGATGCTATTC
Hairpin Sequence:
5'-CCGG-ATGCTCACCTGGATGCTATTC-CTCGAG-GAATAGCATCCAGGTGAGCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGCTCACCTGGATGCTATTCCTCGAGGAATAGCATCCAGGTGAGCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGCTCACCTGGATGCTATTCCTCGAGGAATAGCATCCAGGTGAGCAT-3'

Other clones with same target sequence:

(none)