Construct: shRNA TRCN0000345235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029880.2-654s21c1
DNA Barcode:
GCCATATATCTGCTGGATCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptgr2 (77219)
Gene Description:
prostaglandin reductase 2
Transcript:
RefSeq NM_029880.2 (NON-CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATATATCTGCTGGATCTA
Hairpin Sequence:
5'-CCGG-GCCATATATCTGCTGGATCTA-CTCGAG-TAGATCCAGCAGATATATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATATATCTGCTGGATCTACTCGAGTAGATCCAGCAGATATATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATATATCTGCTGGATCTACTCGAGTAGATCCAGCAGATATATGGC-3'

Other clones with same target sequence:

  1. TRCN0000180686