Construct: shRNA TRCN0000345408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008667.3-1974s21c1
DNA Barcode:
ATTGTAGGCATTCACTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nab1 (17936)
Gene Description:
Ngfi-A binding protein 1
Transcript:
RefSeq NM_008667.3 (CURRENT)
Match location:
Position 1974 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTGTAGGCATTCACTATTTA
Hairpin Sequence:
5'-CCGG-ATTGTAGGCATTCACTATTTA-CTCGAG-TAAATAGTGAATGCCTACAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTGTAGGCATTCACTATTTACTCGAGTAAATAGTGAATGCCTACAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTGTAGGCATTCACTATTTACTCGAGTAAATAGTGAATGCCTACAAT-3'

Other clones with same target sequence:

(none)