Construct: shRNA TRCN0000345431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176996.4-3477s21c1
DNA Barcode:
CCCTACATTCACATCTCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smo (319757)
Gene Description:
smoothened, frizzled class receptor
Transcript:
RefSeq NM_176996.4 (CURRENT)
Match location:
Position 3477 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACATTCACATCTCAGTT
Hairpin Sequence:
5'-CCGG-CCCTACATTCACATCTCAGTT-CTCGAG-AACTGAGATGTGAATGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACATTCACATCTCAGTTCTCGAGAACTGAGATGTGAATGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACATTCACATCTCAGTTCTCGAGAACTGAGATGTGAATGTAGGG-3'

Other clones with same target sequence:

  1. TRCN0000026274