Construct: shRNA TRCN0000345543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025355.3-855s21c1
DNA Barcode:
TAATGCCTTTGGCCATCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tceal6 (66104)
Gene Description:
transcription elongation factor A (SII)-like 6
Transcript:
RefSeq NM_025355.3 (NON-CURRENT)
Match location:
Position 855 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATGCCTTTGGCCATCAATT
Hairpin Sequence:
5'-CCGG-TAATGCCTTTGGCCATCAATT-CTCGAG-AATTGATGGCCAAAGGCATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATGCCTTTGGCCATCAATTCTCGAGAATTGATGGCCAAAGGCATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATGCCTTTGGCCATCAATTCTCGAGAATTGATGGCCAAAGGCATTA-3'

Other clones with same target sequence:

(none)