Construct: shRNA TRCN0000345692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011822.3-1547s21c1
DNA Barcode:
CGCTCTGTACTACCTGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pigq (14755)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis, class Q
Transcript:
RefSeq NM_011822.3 (NON-CURRENT)
Match location:
Position 1547 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCTGTACTACCTGGTATT
Hairpin Sequence:
5'-CCGG-CGCTCTGTACTACCTGGTATT-CTCGAG-AATACCAGGTAGTACAGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCTGTACTACCTGGTATTCTCGAGAATACCAGGTAGTACAGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCTGTACTACCTGGTATTCTCGAGAATACCAGGTAGTACAGAGCG-3'

Other clones with same target sequence:

  1. TRCN0000018429