Construct: shRNA TRCN0000345720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153085.3-2133s21c1
DNA Barcode:
GCGTGAAGAAGCCCATAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wac (225131)
Gene Description:
WW domain containing adaptor with coiled-coil
Transcript:
RefSeq NM_153085.3 (NON-CURRENT)
Match location:
Position 2133 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGAAGAAGCCCATAATAT
Hairpin Sequence:
5'-CCGG-GCGTGAAGAAGCCCATAATAT-CTCGAG-ATATTATGGGCTTCTTCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGAAGAAGCCCATAATATCTCGAGATATTATGGGCTTCTTCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGAAGAAGCCCATAATATCTCGAGATATTATGGGCTTCTTCACGC-3'

Other clones with same target sequence:

(none)