Construct: shRNA TRCN0000345745

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026604.4-5234s21c1
DNA Barcode:
GATCCTTATTCAGACTATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam135a (68187)
Gene Description:
family with sequence similarity 135, member A
Transcript:
RefSeq NM_026604.4 (CURRENT)
Match location:
Position 5234 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345745)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCTTATTCAGACTATATC
Hairpin Sequence:
5'-CCGG-GATCCTTATTCAGACTATATC-CTCGAG-GATATAGTCTGAATAAGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCTTATTCAGACTATATCCTCGAGGATATAGTCTGAATAAGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCTTATTCAGACTATATCCTCGAGGATATAGTCTGAATAAGGATC-3'

Other clones with same target sequence:

(none)