Construct: shRNA TRCN0000345861

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177173.5-1859s21c1
DNA Barcode:
CCTTACTTATTGTCCTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A830018L16Rik (320492)
Gene Description:
RIKEN cDNA A830018L16 gene
Transcript:
RefSeq NM_177173.5 (NON-CURRENT)
Match location:
Position 1859 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345861)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTACTTATTGTCCTAATTT
Hairpin Sequence:
5'-CCGG-CCTTACTTATTGTCCTAATTT-CTCGAG-AAATTAGGACAATAAGTAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTACTTATTGTCCTAATTTCTCGAGAAATTAGGACAATAAGTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTACTTATTGTCCTAATTTCTCGAGAAATTAGGACAATAAGTAAGG-3'

Other clones with same target sequence:

(none)