Construct: shRNA TRCN0000345872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027256.1-880s21c1
DNA Barcode:
CCTGAGAGTATAGTTCCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ints4 (101861)
Gene Description:
integrator complex subunit 4
Transcript:
RefSeq NM_027256.1 (NON-CURRENT)
Match location:
Position 880 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAGTATAGTTCCAATA
Hairpin Sequence:
5'-CCGG-CCTGAGAGTATAGTTCCAATA-CTCGAG-TATTGGAACTATACTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAGTATAGTTCCAATACTCGAGTATTGGAACTATACTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAGTATAGTTCCAATACTCGAGTATTGGAACTATACTCTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000198023