Construct: shRNA TRCN0000345875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024181.2-1124s21c1
DNA Barcode:
GCAGTGAAGTACAATGGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnajc10 (66861)
Gene Description:
DnaJ heat shock protein family (Hsp40) member C10
Transcript:
RefSeq NM_024181.2 (CURRENT)
Match location:
Position 1124 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGAAGTACAATGGAGAT
Hairpin Sequence:
5'-CCGG-GCAGTGAAGTACAATGGAGAT-CTCGAG-ATCTCCATTGTACTTCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGAAGTACAATGGAGATCTCGAGATCTCCATTGTACTTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGAAGTACAATGGAGATCTCGAGATCTCCATTGTACTTCACTGC-3'

Other clones with same target sequence:

  1. TRCN0000111887