Construct: shRNA TRCN0000345919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023268.2-1423s21c1
DNA Barcode:
CATGAGGAGCTATGTTCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qsox1 (104009)
Gene Description:
quiescin Q6 sulfhydryl oxidase 1
Transcript:
RefSeq NM_023268.2 (CURRENT)
Match location:
Position 1423 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAGGAGCTATGTTCAGTT
Hairpin Sequence:
5'-CCGG-CATGAGGAGCTATGTTCAGTT-CTCGAG-AACTGAACATAGCTCCTCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAGGAGCTATGTTCAGTTCTCGAGAACTGAACATAGCTCCTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAGGAGCTATGTTCAGTTCTCGAGAACTGAACATAGCTCCTCATG-3'

Other clones with same target sequence:

  1. TRCN0000177762