Construct: shRNA TRCN0000345932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177815.3-703s21c1
DNA Barcode:
CACAACTGTTGCCCAGTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rft1 (328370)
Gene Description:
RFT1 homolog
Transcript:
RefSeq NM_177815.3 (CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAACTGTTGCCCAGTATTT
Hairpin Sequence:
5'-CCGG-CACAACTGTTGCCCAGTATTT-CTCGAG-AAATACTGGGCAACAGTTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAACTGTTGCCCAGTATTTCTCGAGAAATACTGGGCAACAGTTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAACTGTTGCCCAGTATTTCTCGAGAAATACTGGGCAACAGTTGTG-3'

Other clones with same target sequence:

(none)