Construct: shRNA TRCN0000345996

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024499.1-1692s21c1
DNA Barcode:
GCCACCAGAACCATCTTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgta (52551)
Gene Description:
small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Transcript:
RefSeq NM_024499.1 (NON-CURRENT)
Match location:
Position 1692 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000345996)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACCAGAACCATCTTTGTT
Hairpin Sequence:
5'-CCGG-GCCACCAGAACCATCTTTGTT-CTCGAG-AACAAAGATGGTTCTGGTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACCAGAACCATCTTTGTTCTCGAGAACAAAGATGGTTCTGGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACCAGAACCATCTTTGTTCTCGAGAACAAAGATGGTTCTGGTGGC-3'

Other clones with same target sequence:

  1. TRCN0000189716