Construct: shRNA TRCN0000346054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027335.1-445s21c1
DNA Barcode:
TCGAGCAGGTGCGGGATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2210016F16Rik (70153)
Gene Description:
RIKEN cDNA 2210016F16 gene
Transcript:
RefSeq NM_027335.1 (CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGAGCAGGTGCGGGATATAT
Hairpin Sequence:
5'-CCGG-TCGAGCAGGTGCGGGATATAT-CTCGAG-ATATATCCCGCACCTGCTCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGAGCAGGTGCGGGATATATCTCGAGATATATCCCGCACCTGCTCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGAGCAGGTGCGGGATATATCTCGAGATATATCCCGCACCTGCTCGA-3'

Other clones with same target sequence:

(none)