Construct: shRNA TRCN0000346230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019536.1-3875s21c1
DNA Barcode:
ACGGAGCAAGAACTGGTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dnah10 (56087)
Gene Description:
dynein, axonemal, heavy chain 10
Transcript:
RefSeq NM_019536.1 (CURRENT)
Match location:
Position 3875 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGAGCAAGAACTGGTTAAT
Hairpin Sequence:
5'-CCGG-ACGGAGCAAGAACTGGTTAAT-CTCGAG-ATTAACCAGTTCTTGCTCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGAGCAAGAACTGGTTAATCTCGAGATTAACCAGTTCTTGCTCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGAGCAAGAACTGGTTAATCTCGAGATTAACCAGTTCTTGCTCCGT-3'

Other clones with same target sequence:

(none)