Construct: shRNA TRCN0000346340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163639.1-1413s21c1
DNA Barcode:
GCTGCAAGCGCCTAGTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cfap70 (76670)
Gene Description:
cilia and flagella associated protein 70
Transcript:
RefSeq NM_001163639.1 (CURRENT)
Match location:
Position 1413 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAAGCGCCTAGTATAAA
Hairpin Sequence:
5'-CCGG-GCTGCAAGCGCCTAGTATAAA-CTCGAG-TTTATACTAGGCGCTTGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAAGCGCCTAGTATAAACTCGAGTTTATACTAGGCGCTTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAAGCGCCTAGTATAAACTCGAGTTTATACTAGGCGCTTGCAGC-3'

Other clones with same target sequence:

(none)