Construct: shRNA TRCN0000346424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029022.1-1403s21c1
DNA Barcode:
CATAGTATTAGGTGGTATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scrn3 (74616)
Gene Description:
secernin 3
Transcript:
RefSeq NM_029022.1 (CURRENT)
Match location:
Position 1403 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGTATTAGGTGGTATTTC
Hairpin Sequence:
5'-CCGG-CATAGTATTAGGTGGTATTTC-CTCGAG-GAAATACCACCTAATACTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGTATTAGGTGGTATTTCCTCGAGGAAATACCACCTAATACTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGTATTAGGTGGTATTTCCTCGAGGAAATACCACCTAATACTATG-3'

Other clones with same target sequence:

(none)