Construct: shRNA TRCN0000346442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030197.1-1529s21c1
DNA Barcode:
CCGGACTTCTTTCTTTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cacul1 (78832)
Gene Description:
CDK2 associated, cullin domain 1
Transcript:
RefSeq NM_030197.1 (NON-CURRENT)
Match location:
Position 1529 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGACTTCTTTCTTTGATTT
Hairpin Sequence:
5'-CCGG-CCGGACTTCTTTCTTTGATTT-CTCGAG-AAATCAAAGAAAGAAGTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGACTTCTTTCTTTGATTTCTCGAGAAATCAAAGAAAGAAGTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGACTTCTTTCTTTGATTTCTCGAGAAATCAAAGAAAGAAGTCCGG-3'

Other clones with same target sequence:

  1. TRCN0000191053