Construct: shRNA TRCN0000346487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028096.1-961s21c1
DNA Barcode:
CGCAACCAGCGATCAAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2010300C02Rik (72097)
Gene Description:
RIKEN cDNA 2010300C02 gene
Transcript:
RefSeq NM_028096.1 (CURRENT)
Match location:
Position 961 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAACCAGCGATCAAGTAAA
Hairpin Sequence:
5'-CCGG-CGCAACCAGCGATCAAGTAAA-CTCGAG-TTTACTTGATCGCTGGTTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAACCAGCGATCAAGTAAACTCGAGTTTACTTGATCGCTGGTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAACCAGCGATCAAGTAAACTCGAGTTTACTTGATCGCTGGTTGCG-3'

Other clones with same target sequence:

(none)