Construct: shRNA TRCN0000346660

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001163103.1-1032s21c1
DNA Barcode:
CCCGAGAGGAGAACATAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp1r36 (210762)
Gene Description:
protein phosphatase 1, regulatory subunit 36
Transcript:
RefSeq NM_001163103.1 (CURRENT)
Match location:
Position 1032 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346660)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGAGGAGAACATAATAA
Hairpin Sequence:
5'-CCGG-CCCGAGAGGAGAACATAATAA-CTCGAG-TTATTATGTTCTCCTCTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGAGGAGAACATAATAACTCGAGTTATTATGTTCTCCTCTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGAGGAGAACATAATAACTCGAGTTATTATGTTCTCCTCTCGGG-3'

Other clones with same target sequence:

(none)