Construct: shRNA TRCN0000346722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173758.3-3262s21c1
DNA Barcode:
CTGACTCTTAGACGCTGATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vwa8 (219189)
Gene Description:
von Willebrand factor A domain containing 8
Transcript:
RefSeq NM_173758.3 (CURRENT)
Match location:
Position 3262 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACTCTTAGACGCTGATAG
Hairpin Sequence:
5'-CCGG-CTGACTCTTAGACGCTGATAG-CTCGAG-CTATCAGCGTCTAAGAGTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACTCTTAGACGCTGATAGCTCGAGCTATCAGCGTCTAAGAGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACTCTTAGACGCTGATAGCTCGAGCTATCAGCGTCTAAGAGTCAG-3'

Other clones with same target sequence:

(none)