Construct: shRNA TRCN0000346791

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026605.2-2055s21c1
DNA Barcode:
TCAGGCACTCTGGACAAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sympk (68188)
Gene Description:
symplekin
Transcript:
RefSeq NM_026605.2 (CURRENT)
Match location:
Position 2055 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346791)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGGCACTCTGGACAAATAT
Hairpin Sequence:
5'-CCGG-TCAGGCACTCTGGACAAATAT-CTCGAG-ATATTTGTCCAGAGTGCCTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGGCACTCTGGACAAATATCTCGAGATATTTGTCCAGAGTGCCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGGCACTCTGGACAAATATCTCGAGATATTTGTCCAGAGTGCCTGA-3'

Other clones with same target sequence:

(none)