Construct: shRNA TRCN0000346849

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001182.3-6449s21c1
DNA Barcode:
CCTGTGAACTTGAAACTCGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Baz2b (407823)
Gene Description:
bromodomain adjacent to zinc finger domain, 2B
Transcript:
RefSeq NM_001001182.3 (CURRENT)
Match location:
Position 6449 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346849)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGAACTTGAAACTCGTA
Hairpin Sequence:
5'-CCGG-CCTGTGAACTTGAAACTCGTA-CTCGAG-TACGAGTTTCAAGTTCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGAACTTGAAACTCGTACTCGAGTACGAGTTTCAAGTTCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGAACTTGAAACTCGTACTCGAGTACGAGTTTCAAGTTCACAGG-3'

Other clones with same target sequence:

  1. TRCN0000192271