Construct: shRNA TRCN0000346880

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177805.3-5973s21c1
DNA Barcode:
GAAGTGGAAGAAACGTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Togaram1 (328108)
Gene Description:
TOG array regulator of axonemal microtubules 1
Transcript:
RefSeq NM_177805.3 (CURRENT)
Match location:
Position 5973 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000346880)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGGAAGAAACGTCATTT
Hairpin Sequence:
5'-CCGG-GAAGTGGAAGAAACGTCATTT-CTCGAG-AAATGACGTTTCTTCCACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGGAAGAAACGTCATTTCTCGAGAAATGACGTTTCTTCCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGGAAGAAACGTCATTTCTCGAGAAATGACGTTTCTTCCACTTC-3'

Other clones with same target sequence:

(none)