Construct: shRNA TRCN0000347150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001111282.1-1025s21c1
DNA Barcode:
GTTGGTGCACAGCACAAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgb1-ps7 (434174)
Gene Description:
high-mobility group high mobility group box 1, pseudogene 7
Transcript:
RefSeq NM_001111282.1 (NON-CURRENT)
Match location:
Position 1025 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTGGTGCACAGCACAAATTA
Hairpin Sequence:
5'-CCGG-GTTGGTGCACAGCACAAATTA-CTCGAG-TAATTTGTGCTGTGCACCAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTGGTGCACAGCACAAATTACTCGAGTAATTTGTGCTGTGCACCAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTGGTGCACAGCACAAATTACTCGAGTAATTTGTGCTGTGCACCAAC-3'

Other clones with same target sequence:

(none)