Construct: shRNA TRCN0000347392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081235.1-2810s21c1
DNA Barcode:
CCGGGACCTTCTTCGACAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mn1 (433938)
Gene Description:
meningioma 1
Transcript:
RefSeq NM_001081235.1 (CURRENT)
Match location:
Position 2810 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGACCTTCTTCGACAAAT
Hairpin Sequence:
5'-CCGG-CCGGGACCTTCTTCGACAAAT-CTCGAG-ATTTGTCGAAGAAGGTCCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGACCTTCTTCGACAAATCTCGAGATTTGTCGAAGAAGGTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGACCTTCTTCGACAAATCTCGAGATTTGTCGAAGAAGGTCCCGG-3'

Other clones with same target sequence:

(none)