Construct: shRNA TRCN0000347431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030110.1-1102s21c1
DNA Barcode:
GAATTTCTTTCCTACTCTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Micu3 (78506)
Gene Description:
mitochondrial calcium uptake family, member 3
Transcript:
RefSeq NM_030110.1 (CURRENT)
Match location:
Position 1102 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000347431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATTTCTTTCCTACTCTAAT
Hairpin Sequence:
5'-CCGG-GAATTTCTTTCCTACTCTAAT-CTCGAG-ATTAGAGTAGGAAAGAAATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATTTCTTTCCTACTCTAATCTCGAGATTAGAGTAGGAAAGAAATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATTTCTTTCCTACTCTAATCTCGAGATTAGAGTAGGAAAGAAATTC-3'

Other clones with same target sequence:

(none)